Journal: Frontiers in Pharmacology

Article Title: Brg1 and RUNX1 synergy in regulating TRPM4 channel in mouse cardiomyocytes

doi: 10.3389/fphar.2024.1494205

Figure Lengend Snippet: Brg1 modulated TRPM4 promoter activity by interaction with RUNX1. (A) Predicting the residue label and hydrogen bond length of interaction between Brg1 and RUNX1, and the combination state and surface structure of Brg1 and RUNX1 by Protein Data Bank. (B) Co-immunoprecipitation assays of Brg1 and RUNX1 on the nucleoprotein extracted from neonatal mouse cardiomyocytes. (C) RUNX1 significantly boosted TRPM4 promoter activity. Statistical analysis was performed with two-tailed Student’s t-test. * p < 0.05, ** p < 0.01 vs. NC group. n = 8 in each group. (D) Brg1 knockdown significantly decreased the TRPM4 promoter activity. ** p < 0.01 vs. Scramble-siRNA group by two-tailed Student’s t-test. n = 6 in each group. (E) Inhibited Brg1 by PFI-3 (10 μM) significantly reduced the TRPM4 promoter activity. ** p < 0.01 vs. DMSO group by two-tailed Student’s t-test. n = 6 in each group. (F) RUNX1 knockdown declined the increases in TRPM4 promoter activity caused by Brg1-OE. * p < 0.05, vs. NC group; ## p < 0.01 vs. +Scramble-siRNA by one-way ANOVA analysis. n = 6 in each group. (G) A ChIP assay showed the binding site of RUNX1 an s c2d TRPM4 promoter. (H) qRT-PCR of the ChIP products demonstrated that both Brg1 and RUNX1 were bound to the TRPM4 promoter region, IgG served as an antibody control. ** p < 0.01 and *** p < 0.001 vs. IgG group by a two-tailed Student’s t-test. n = 3 in each group.

Article Snippet: After incubation in 5% non-fat milk for 1.5 h at room temperature, the membranes were incubated at 4°C overnight with TRPM4 antibody (1:500; ABclonal Technology Co.,Ltd.), β-actin antibody (1:1000; Santa, United States), Brg1 antibody (Sigma-Aldrich, 07–478, 1:500) and RUNX1 antibody (Proteintech 25315-1-AP, 1:1000).

Techniques: Activity Assay, Residue, Immunoprecipitation, Two Tailed Test, Knockdown, Binding Assay, Quantitative RT-PCR, Control

Journal: Frontiers in Pharmacology

Article Title: Brg1 and RUNX1 synergy in regulating TRPM4 channel in mouse cardiomyocytes

doi: 10.3389/fphar.2024.1494205

Figure Lengend Snippet: A schematic diagram summarizing the potential mechanisms that Brg1 interacted with RUNX1 transcriptional regulated TRPM4, that eventually leads to TRPM4 overactivation in cardiomyocytes induced by hypoxia.

Article Snippet: After incubation in 5% non-fat milk for 1.5 h at room temperature, the membranes were incubated at 4°C overnight with TRPM4 antibody (1:500; ABclonal Technology Co.,Ltd.), β-actin antibody (1:1000; Santa, United States), Brg1 antibody (Sigma-Aldrich, 07–478, 1:500) and RUNX1 antibody (Proteintech 25315-1-AP, 1:1000).

Techniques:

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: The effects of peptide targeted FLT-4 in leukemic mouse model. (A) Expressions of NK cells and IFN-γ of NK cells in BM after single, double, and triple treatments (n=12). (B) Expressions of NK cells and IFN-γ of NK cells in spleen after single, double, and triple treatments (n=12). (C) Expressions of NK cells and IFN-γ of NK cells in liver after single, double, and triple treatments. Bars represent an average (mean)±SE and Asterisks depict statistically significant differences compared to C1498 injected groups (*p<0.05 vs. C1498 injected group, n=12). WT: wild type, C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Injection

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: The effects of peptide targeted FLT-4 in AML mouse model. The transcriptional level of cytolytic factors including LAMP1, perforin, granzyme B, and IFN-γ displayed in spleen and liver. Bars represent an average (mean)±SE and Asterisks depict statistically significant differences compared to C1498 injected groups. Data shown represent the means of independent experiment with duplicate (*p<0.05 vs. C1498 injected group, n=4). WT: wild type, C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Injection

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: Combinational therapies with peptide and ara-C effectively suppress leukemia blast cells in AML mice. (A) H&E staining. BM, spleen and liver from C1498 cells injection mice showed successful engraftment of leukemia cells. Insets (magnification, ×40) show enlargement of cells within the main images (magnification, ×10). (B) Statistical analysis for the upper panel of a single treated group. And the lower panel for double-, triple treatment groups. Bars represent an average (mean)±SE, and asterisks represent statistically significant differences compared to C1498 injected group The indicated values are the averages calculated from at least 5 randomly selected fields of each group of 3 independent experiments (*p<0.05; **p<0.01; vs. C1498 injected group, n=12). C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Staining, Injection

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: Comparison of survival over time in the groups. No significant differences in the survival rate were detected between each group, however, dual and triple treated groups displayed a long life span, compared to that of C1498 groups and single only treated groups. It suggests the benefits of peptide and ara-C in AML treatment. WT: wild type, C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Comparison, Injection

Journal: International Journal of Molecular Sciences

Article Title: Novel Benzoxazoles Containing 4-Amino-Butanamide Moiety Inhibited LPS-Induced Inflammation by Modulating IL-6 or IL-1β mRNA Expression

doi: 10.3390/ijms23105331

Figure Lengend Snippet: Compounds 5f and 4d attenuate inflammation in vitro. Effects of compounds (10 µM) on the protein levels of STAT3, IκB, and NF-κB in AML-12 cells. ( a ) Expression levels of inflammation related-proteins in vitro. ( b – d ) Protein expression levels were normal-ized against the indicated protein. * p < 0.05 compared with the group treated with the PBS (vehicle). + p < 0.05 compared with the group treated with the LPS. Data are pre-sented as the mean ± SD.

Article Snippet: The human keratinocytes HaCaT or the alpha mouse liver 12 (AML-12) cells were obtained from Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: In Vitro, Expressing

Journal: Oncology Letters

Article Title: Circular RNA ATAD1 is upregulated in acute myeloid leukemia and promotes cancer cell proliferation by downregulating miR-34b via promoter methylation

doi: 10.3892/ol.2021.13060

Figure Lengend Snippet: Circ-ATAD1 overexpression increases acute myelogenous leukemia cell proliferation through miR-34b. Role of circ-ATAD1 and miR-34b in regulating (A) Kasumi-3 and (B) Kasumi-6 cell proliferation was analyzed by BrdU assay. Representative images of (C) Kasumi-3 and (D) Kasumi-6 cells are displayed. Scale bar, 20 µm. Blue signals indicate DAPI staining; red signals indicate Brdu signaling Normalized data of three biological replicates are presented. Mean values of three replicates are presented. *P<0.05. circ, circular RNA; miR, microRNA; NC, negative control.

Article Snippet: Kasumi-3 and Kasumi-6 AML cell lines were purchased from the ATCC, and cultured in RPMI-1640 medium (10% FBS) at 37°C in an incubator with 5% CO 2 and 95% humidity.

Techniques: Over Expression, BrdU Staining, Staining, Negative Control